Deep mutational scanning of H5 hemagglutinin to inform influenza virus surveillance
The Story So Far
Short impact summary
The study "Deep mutational scanning of H5 hemagglutinin to inform influenza virus surveillance" has garnered significant attention, with 79 citations since publication. The research is grounded in prominent institutions such as St. Jude Children's Research Hospital and the University of Washington, and has also received robust financial backing from entities like the NIH and the Biotechnology and Biological Sciences Research Council.
Full narrative
The research article titled "Deep mutational scanning of H5 hemagglutinin to inform influenza virus surveillance" has emerged as a pivotal contribution to the field of virology, particularly in understanding influenza virus dynamics. Since its release, the study has received considerable academic acknowledgment, evidenced by a citation count of 79, indicating its relevance to ongoing conversations in the scientific community. The work was supported by esteemed institutions like St. Jude Children's Research Hospital, the University of Washington, and Howard Hughes Medical Institute, underscoring the collaborative efforts driving this vital research.
One of the hallmarks of this research is its commitment to open science practices, exemplified by the early dissemination of findings through a preprint version. This preprint effectively primed the community for subsequent discussion and engagement, accumulating 16 citations and generating four early sharing events. Such contributions reflect a proactive approach to science, facilitating immediate discourse and collaboration prior to formal publication in a peer-reviewed venue like PLoS Biology.
Furthermore, the work is reinforced by substantial financial investment from key funding agencies, including the National Institute of Health and the Biotechnology and Biological Sciences Research Council. This financial backing not only validates the significance of the research but also reflects a broader institutional interest in enhancing influenza surveillance methodologies. The multi-faceted support reveals an extensive commitment to advancing public health through sound scientific research.
In terms of community engagement, while the study has yet to generate significant online discussion on platforms like Wikipedia or Reddit, it has inspired dialogues within the academic sphere, with a noted presence in reputable venues such as bioRxiv and npj Viruses. This indicates the study's potential to influence ongoing research and discourse surrounding influenza virus surveillance. Additionally, the use of resources like Zenodo and GitHub for data sharing exemplifies the authors' commitment to enhancing the reproducibility and accessibility of their research, allowing fellow scientists to build upon their findings effectively.
Impact metrics
-
Total Citations (OpenAlex):
79
🏆 Top 1% Cited
Includes 63 from the published article and 16 from its early preprint version.
-
Total Online mentions (Crossref & Open Web): 2
Includes 0 from the published article and 4 from the preprint.
- Citation context (scite): 19
Inferred roles
Heuristic classification from citation composition / usage signals. Not based on full text.
High-visibility uptake (0.85)
Scholarly uptake (0.73)
Sustainability & policy relevance (0.55)
Rapid uptake (0.46)
Active public discourse (0.42)
Access OpenAlex
- DOI: 10.1371/journal.pbio.3002916
- OA status: Gold
- Landing page: https://doi.org/10.1371/journal.pbio.3002916
- Best OA PDF: https://journals.plos.org/plosbiology/article/file?id=10.1371/journal.pbio.3002916&type=printable
Attention landscape Event Data
This work has 2 recorded events globally (combined from the published article and preprint).
(See specific sources and examples in the Online Mentions section below).
Citation context scite
- Total: 19
- Supporting: 1
- Mentioning: 18
- Contradicting: 0
Scientific Stewardship Europe PMC / DataCite
Associated data repositories and clinical trials.
Data Repositories & Code
Who is citing this work? OpenAlex
Analysis of the 79 most recent citing works.
101.6% of these citations come from the Top 10 institutions below. The median citing paper has gone on to be cited 1.0 times itself.
101.6% of these citations come from the Top 10 institutions below. The median citing paper has gone on to be cited 1.0 times itself.
Global Reach (Top Countries)
United States (40)
Canada (6)
Netherlands (6)
South Africa (6)
India (5)
United Kingdom (4)
Hong Kong (4)
China (4)
Japan (3)
Switzerland (2)
Belgium (2)
Lithuania (1)
Institutional Sectors
Education (53)
Nonprofit (24)
Facility (22)
Healthcare (15)
Government (9)
Top Citing Institutions
- University of Washington ROR (12)
- Fred Hutch Cancer Center ROR (12)
- Howard Hughes Medical Institute ROR (10)
- University of Pennsylvania ROR (5)
- Utrecht University ROR (5)
- Cape Town HVTN Immunology Laboratory / Hutchinson Centre Research Institute of South Africa ROR (5)
- Scripps Research Institute ROR (4)
- University of Georgia ROR (4)
- University of Hong Kong ROR (4)
- Centers for Disease Control and Prevention ROR (3)
- Citing work types: article (34) preprint (18) review (7) letter (3) editorial (1)
- Top citing venues: bioRxiv (17) bioRxiv (Cold Spring Harbor Laboratory) (5) npj Viruses (3) Nature (2) Nature Communications (2)
- Top citing concepts: Virology (31) Influenza A virus subtype H5N1 (26) Biology (25) Hemagglutinin (influenza) (14) Computational biology (6) Virus (4) Clade (4) Mutation (3)
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Citation timeline:
Online mentions & activity Crossref & Open Web
Community Engagement & Discourse
Bluesky Post
2024-11-22
"Pseudovirus deep mutational scanning reveals mutations in 2.3.4.4b H5 HA affect phenotype, e.g. binding to α2-6-linked sialic acids, HA stabilisation,..." — @duckswabber.bsky.social
View Thread ↗
Displaying the 1 most detailed mentions. Other aggregated events lack public URLs.
Contributors & affiliations OpenAlex
15 authors · 7 institutions
Authors
- Bernadeta Dadonaite First Corresponding ORCID
- Jenny Ahn Corresponding ORCID
- Jordan T. Ort Corresponding ORCID
- Yu Jin Corresponding ORCID
- Colleen Furey Corresponding ORCID
- Annie Dosey Corresponding ORCID
- William W. Hannon Corresponding ORCID
- Amy L. Vincent Baker Corresponding
- Richard J. Webby Corresponding
- Neil P. King Corresponding ORCID
- Yan Liu Corresponding ORCID
- Scott E. Hensley Corresponding ORCID
- Thomas P. Peacock Corresponding ORCID
- Louise H. Moncla Corresponding ORCID
- Jesse D. Bloom Corresponding ORCID
Institutions
Concepts & topics OpenAlex
13 specific concepts identified.
Biology
Hemagglutinin (influenza)
Virology
Virus
Mutation
Influenza A virus
Neutralization
Phenotype
Antigenic drift
Mutagenesis
Sialic acid
Gene
Genetics
UN Sustainable Development Goals OpenAlex
1 targets detected.
Good health and well-being (0.85)
Research Funding Europe PMC / OpenAlex
19 financial grants and sponsors detected.
Biotechnology and Biological Sciences Research Council
(BBS/E/PI/230002B)
NIAID NIH HHS
(R01 AI141707)
National Institute of Health
(S10-OD-020069)
NIAID NIH HHS
(75N93021C00015)
Howard Hughes Medical Institute
Division of Intramural Research, National Institute of Allergy and Infectious Diseases
(AI47029)
Biotechnology and Biological Sciences Research Council
(BBS/E/PI/230002A; BBS/E/PI/230002B)
Division of Intramural Research, National Institute of Allergy and Infectious Diseases
(R01AI141707)
NIH HHS
(S10 OD028685)
Biotechnology and Biological Sciences Research Council
(BB/Y007298/1)
Medical Research Council
(MR/R010757/1)
NIH HHS
(S10 OD020069)
Open Philanthropy Project
NIAID NIH HHS
(R00 AI147029)
NIAID NIH HHS
(R01 AI165821)
Division of Intramural Research, National Institute of Allergy and Infectious Diseases
(R01AI165821)
Division of Intramural Research, National Institute of Allergy and Infectious Diseases
(75N93021C00015)
Medical Research Foundation
(MR/Y03368X/1)
National Institute of Health
(S10-OD-028685)
Citations OpenAlex
Displaying 63 most recent citing works
| Year | Title | Venue | DOI |
|---|---|---|---|
| 2024 | The global H5N1 influenza panzootic in mammals | Nature | 10.1038/s41586-024-08054-z |
| 2024 | A single mutation in bovine influenza H5N1 hemagglutinin switches specificity to human receptors | Science | 10.1126/science.adt0180 |
| 2024 | Critical Illness in an Adolescent with Influenza A(H5N1) Virus Infection | New England Journal of Medicine | 10.1056/nejmc2415890 |
| 2024 | A single mutation in dairy cow-associated H5N1 viruses increases receptor binding breadth | Nature Communications | 10.1038/s41467-024-54934-3 |
| 2025 | Receptor-binding specificity of a bovine influenza A virus | Nature | 10.1038/s41586-025-08822-5 |
| 2025 | H5N1 2.3.4.4b: a review of mammalian adaptations and risk of pandemic emergence | Journal of General Virology | 10.1099/jgv.0.002109 |
| 2025 | Multiplexed assays of variant effect for clinical variant interpretation | Nature Reviews Genetics | 10.1038/s41576-025-00870-x |
| 2025 | Large-scale computational modelling of H5 influenza variants against HA1-neutralising antibodies | EBioMedicine | 10.1016/j.ebiom.2025.105632 |
| 2025 | Strategies and efforts in circumventing the emergence of antiviral resistance against conventional antivirals | npj Antimicrobials and Resistance | 10.1038/s44259-025-00125-z |
| 2025 | Structure of a zoonotic H5N1 hemagglutinin reveals a receptor-binding site occupied by an auto-glycan | Structure | 10.1016/j.str.2025.01.001 |
| 2025 | Deep mutational scanning of rabies glycoprotein defines mutational constraint and antibody-escape mutations | Cell Host & Microbe | 10.1016/j.chom.2025.04.018 |
| 2025 | Pleiotropic mutational effects on function and stability constrain the antigenic evolution of influenza haemagglutinin | Nature Ecology & Evolution | 10.1038/s41559-025-02895-1 |
| 2025 | Attachment and replication of clade 2.3.4.4b influenza A (H5N1) viruses in human respiratory epithelium: an in-vitro study | The Lancet Microbe | 10.1016/j.lanmic.2025.101230 |
| 2025 | Balancing stability and function: impact of the surface charge of SARS-CoV-2 Omicron spike protein | npj Viruses | 10.1038/s44298-025-00104-1 |
| 2025 | The sweet side of H5N1 influenza virus infection | PLoS Pathogens | 10.1371/journal.ppat.1012847 |
| 2025 | The Q226L mutation can convert a highly pathogenic H5 2.3.4.4e virus to bind human-type receptors | Proceedings of the National Academy of Sciences | 10.1073/pnas.2419800122 |
| 2025 | Cross-neutralizing and potent human monoclonal antibodies against historical and emerging H5Nx influenza viruses | Nature Microbiology | 10.1038/s41564-025-02137-x |
| 2025 | Mutations in the influenza virus, primarily H5N1, enhance the virus’s virulence, favor receptor interaction, and increase drug resistance | International Journal of Surgery | 10.1097/js9.0000000000002403 |
| 2025 | Impact of naturally occurring hemagglutinin substitutions on antigenicity and fitness of influenza A(H5N1) virus | npj Viruses | 10.1038/s44298-025-00154-5 |
| 2025 | Development of avian influenza A(H5) virus datasets for Nextclade enables rapid and accurate clade assignment | Virus Evolution | 10.1093/ve/veaf058 |
| 2026 | Management of critical illness in an adolescent caused by highly pathogenic avian influenza A(H5N1) virus infection in British Columbia, Canada | The Lancet Infectious Diseases | 10.1016/s1473-3099(25)00773-x |
| 2026 | Pathogenesis of H5N1 Clade 2.3.4.4b in dry Jersey cows following intramammary inoculation shows within-host compartmentalization | bioRxiv (Cold Spring Harbor Laboratory) | 10.64898/2026.03.04.709389 |
| 2024 | Analysis of the Monophyletic Lineage of Avian Influenza H5N1 Which Circulated in Venezuelan Birds During the 2022–2023 Outbreak | Microorganisms | 10.3390/microorganisms12122519 |
| 2025 | Decoding non-human mammalian adaptive signatures of 2.3.4.4b H5N1 to assess its human adaptive potential | Microbiology Spectrum | 10.1128/spectrum.00948-25 |
| 2025 | Nonviral protein cages as tools to decipher and combat viral threats | npj Viruses | 10.1038/s44298-025-00127-8 |
| 2025 | Risk assessment of 2024 cattle H5N1 using age-stratified serosurveillance data | Emerging Microbes & Infections | 10.1080/22221751.2025.2497304 |
| 2025 | A review of the animal influenza viruses and their impact on human health | Discover Public Health | 10.1186/s12982-025-00679-3 |
| 2024 | Receptor Binding Specificity of a Bovine A(H5N1) Influenza Virus | bioRxiv | 10.1101/2024.07.30.605893 |
| 2025 | Pleiotropic mutational effects on function and stability constrain the antigenic evolution of influenza hemagglutinin | bioRxiv | 10.1101/2025.05.24.655919 |
| 2025 | Susceptibility of bovine respiratory and mammary epithelial cells to avian and mammalian derived clade 2.3.4.4b H5N1 highly pathogenic avian influenza viruses | bioRxiv | 10.1101/2025.01.09.632235 |
| 2024 | Deep mutational scanning of rabies glycoprotein defines mutational constraint and antibody-escape mutations | bioRxiv | 10.1101/2024.12.17.628970 |
| 2025 | H5N1 Influenza A is now promiscuous in host range and has improved replication in mammals | bioRxiv | 10.1101/2025.03.15.641219 |
| 2025 | Evaluating variant effect prediction across viruses | bioRxiv | 10.1101/2025.08.04.668549 |
| 2025 | The Q226L mutation can convert a highly pathogenic H5 2.3.4.4e virus to bind human-type receptors | bioRxiv | 10.1101/2025.01.10.632119 |
| 2025 | Seasonal influenza viruses show distinct adaptive dynamics during growth in chicken eggs | bioRxiv | 10.1101/2025.06.12.659400 |
| 2024 | Structure of a zoonotic H5N1 hemagglutinin reveals a receptor-binding site occupied by an auto-glycan | bioRxiv | 10.1101/2024.12.06.626699 |
| 2024 | PLOS Biology and the life sciences in 2024 | PLoS Biology | 10.1371/journal.pbio.3002985 |
| 2024 | Risk assessment of 2024 cattle H5N1 using age-stratified serosurveillance data | bioRxiv | 10.1101/2024.12.23.24319580 |
| 2024 | Predictive Modeling of Respiratory Virus Evolution: Current Capabilities and Limitations | Mathematical Biology and Bioinformatics | 10.17537/2024.19.579 |
| 2025 | A Fine-tuned ProtGPT2 (transformer model) for Predicting more Virulent SARS-CoV-2 variants and understanding its virulence by biophysical methods | bioRxiv | 10.1101/2025.01.13.632691 |
| 2025 | Lengthy delays in H5N1 genome submissions to GISAID | Nature Biotechnology | 10.1038/s41587-025-02636-6 |
| 2025 | Preclinical Evaluation of an mRNA Vaccine Developed from the First Human Isolate of Bovine H5N1 | SSRN Electronic Journal | 10.2139/ssrn.5353082 |
| 2025 | Random mutagenesis of influenza hemagglutinin identifies new sites which modulate its acid-stability and cleavability | bioRxiv | 10.1101/2025.07.25.666873 |
| 2025 | Stabilization of H5 highly pathogenic avian influenza hemagglutinin improves vaccine-elicited neutralizing antibody responses | bioRxiv | 10.1101/2025.07.30.667762 |
| 2025 | Insights from deep mutational scanning in the context of an emerging pathogen | Biochemical Society Transactions | 10.1042/bst20253033 |
| 2025 | Host Switching Mutations in H5N1 Influenza Hemagglutinin Suppress Site-specific Activation Dynamics | bioRxiv | 10.1101/2025.10.06.680362 |
| 2025 | Targeted tiled amplicon based protocol for sequencing the Hemagglutinin (HA) gene segment of seasonal influenza A and influenza B virus from wastewater at high depth of coverage | bioRxiv | 10.1101/2025.10.15.25338105 |
| 2025 | Functional and antigenic constraints on the Nipah virus fusion protein | bioRxiv | 10.1101/2025.10.15.682664 |
| 2025 | Inverted H1 hemagglutinin nanoparticle vaccines protect mice against challenges with human H1N1 and bovine H5N1 influenza viruses | npj Vaccines | 10.1038/s41541-025-01276-w |
| 2025 | AbAgym: a well-curated dataset for the mutational analysis of antibody–antigen complexes | mAbs | 10.1080/19420862.2025.2592421 |
| 2025 | Mutational spectra reveal influenza virus transmission routes and adaptation | bioRxiv | 10.1101/2025.11.26.690773 |
| 2025 | Learning a viral protein’s vocabulary | Nature Ecology & Evolution | 10.1038/s41559-025-02928-9 |
| 2025 | Host switching mutations in H5N1 influenza hemagglutinin suppress site-specific activation dynamics | Nature Communications | 10.1038/s41467-025-66926-y |
| 2025 | Hemagglutinin-displaying influenza nanovaccines: progress and promise | Nanomedicine | 10.1080/17435889.2025.2598329 |
| 2025 | Immunological and virological questions for H5N1 pandemic emergence | ImmunoHorizons | 10.1093/immhor/vlaf062 |
| 2025 | Experimental and computational approaches to adaptive viral evolution: Linking molecular variation to phenotypic outcomes | Journal of Microbiological Methods | 10.1016/j.mimet.2025.107379 |
| 2026 | Influenza hemagglutinin subtypes have different sequence constraints despite sharing extremely similar structures | bioRxiv (Cold Spring Harbor Laboratory) | 10.64898/2026.01.05.697808 |
| 2026 | Loss of α2,3-linked sialoside in the receptor-binding site of a H5N1 influenza hemagglutinin identified in a human patient | bioRxiv (Cold Spring Harbor Laboratory) | 10.64898/2026.01.19.700419 |
| 2026 | Clonal interference and changing selective pressures shape the escape of SARS-CoV-2 from hundreds of antibodies | Virus Evolution | 10.1093/ve/veaf104 |
| 2026 | Functional and antigenic constraints on the Nipah virus fusion protein | Proceedings of the National Academy of Sciences | 10.1073/pnas.2529505123 |
| 2026 | Stabilization of the H5 clade 2.3.4.4b hemagglutinin improves vaccine-elicited neutralizing antibody responses in mice | Science Translational Medicine | 10.1126/scitranslmed.aea8770 |
| 2026 | Evaluation of an H5 influenza virus mRNA-lipid nanoparticle (LNP) vaccine in lactating dairy cows | bioRxiv (Cold Spring Harbor Laboratory) | 10.64898/2026.03.03.709308 |
| 2026 | H5N1 2.3.4.4b HA E190D and Q226H mutations, picked up as minority variants in a patient, result in an inability to bind sialic acid | bioRxiv (Cold Spring Harbor Laboratory) | 10.64898/2026.03.06.710037 |